LOCUS       V003730                 7063 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003730
VERSION     V003730
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Okamoto S, Niki H.
  TITLE     NBRP cloning vector collection sequence project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2017) Contact:Sho Okamoto National Institute of
            Genetics, Microbial Genetics Laboratory; 1111 Yata, Mishima,
            Shizuoka 411-8540, Japan URL
            :https://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE   3  (bases 1 to 7063)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7063)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-APR-2017) Contact:Sho Okamoto National Institute of Genetics,
            Microbial Genetics Laboratory; 1111 Yata, Mishima, Shizuoka
            411-8540, Japan URL :https://www.nig.ac.jp/labs/MicroGen/index.html"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1260..1907
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2663..2677)
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             3773..3943
                     /label="ProtA"
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    4468..4523
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        4997..5101
                     /label="AmpR promoter"
     CDS             5102..5959
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6133..6721
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gatcaatttc ttttaagtaa tctaaatccc cattttttaa tttcttttta gcctctttaa
       61 ataatcctga ataaactaat acctgtttac ctttaagtga tttataaaat gcatcaaaga
      121 ctttttgatt tatttattaa ataatcacta tctttaccag aatacttagc catttcatat
      181 aattctttat tattattttg tcttattttt tgaacttgaa cttgtgttat ttctgaaatg
      241 cccgttacat cacgccataa atctaaccat tcttgttggc taatataata tcttttatct
      301 gtgaaatacg atttatttac tgcaattaac acatgaaaat gaggattata atcatctctt
      361 tttttattat atgtaatctc taacttacga acatatccct ttataacact acctactttt
      421 tttctcttta taagttttct aaaagaatta ttataacgtt ttatttcatt ttctaattca
      481 tcactcatta cattaggtgt agtcaaagtt aaaaagataa actccttttt ctcttgctgc
      541 ttaatatatt gcatcatcaa agataaaccc aatgcatctt ttctagcttt tctccaagca
      601 cagacaggac aaaatcgatt tttacaagaa ttagctttat ataatttctg tttttctaaa
      661 gttttatcag ctacaaaaga cagaaatgta ttgcaatctt caactaaatc catttgattc
      721 tctccaatat gacgtttaat aaatttctga aatacttgat ttctttgttt tttctcagta
      781 tacttttcca tgttataaca cataaaaaca acttagtttt cacaaactat gacaataaaa
      841 aaagttgctt tttccccttt ctatgtatgt tttttactag tcatttaaaa cgatacatta
      901 ataggtacga aaaagcaact ttttttgcgc ttaaaaccag tcataccaat aacttaaggg
      961 taactagcct cgccggcaat agttaccctt attatcaaga taagaaagaa aaggattttt
     1021 cgctacgctc aaatccttta aaaaaacaca aaagaccaca ttttttaatg tggtctttat
     1081 tcttcaacta aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt
     1141 aaaatatata tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag
     1201 aaagcagaca agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa
     1261 tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
     1321 tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
     1381 acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
     1441 taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
     1501 ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
     1561 ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
     1621 aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
     1681 tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
     1741 attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
     1801 taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
     1861 tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa
     1921 tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc gttagttgaa
     1981 gaaggttttt atattacagc tccagatcca tatccttctt tttctgaacc gacttctcct
     2041 ttttcgcttc tttattccaa ttgctttatt gacgttgagc ctcggaaccc ttaacaatcc
     2101 caaaacttgt cgaatggtcg gcttaatagc tcacgctatg ccgacattcg tctgcaagtt
     2161 tagttaaggg ttcttctcaa cgcacaataa attttctcgg cataaatgcg tggtctaatt
     2221 tttattttta ataaccttga tagcaaaaaa tgccattcca atacaaaacc acatacctat
     2281 aatcgataac cacataacag tcataaaacc actccttttt aacaaacttt atcacaagaa
     2341 atatttaaat tttaaatgcc tttattttga attttaaggg gcattttaaa gatttagggg
     2401 taaatcatat agttttatgc ctaaaaacct acagaagctt ttaaaaagca aatatgagcc
     2461 aaataaatat attctaattc tacaaacaaa aatttgagca aattcagtgt cgatttttta
     2521 agacactgcc cagttacatg caaattaaaa ttttcatgat tttttatagt tcctaacagg
     2581 gttaaaattt gtataacgaa agtataatgt ttatataacg ttagtataat aaagcatttt
     2641 aacattatac ttttgataat cgtttatcgt cgtcatcaca ataactttta aaatactcgt
     2701 gcataattca cgctgacctc ccaataacta catggtgtta tcgggaggtc agcacttgtg
     2761 ttttctattt tttaataaaa cctcagcaca taatgaacaa ctttctattt tctatatcac
     2821 ttaaaaccat ttccgaaatt aaacctcagc acattcaaag ccccacttta ttcttaaaaa
     2881 tattttttaa ctcatatgta ttaaaccgct ttcattataa aaaatatctc tatattttat
     2941 ctgtttttat taatcgaaat agcgtgattt tgcggtttta agccttttac ttcctgaata
     3001 aatctttcag caaaatattt attttataag ttgtaaaact tacatttaaa tttaattata
     3061 aatatagatt ttagtattgc aatacataat tcgttatatt atgatgactt tacaaataca
     3121 tacagggggt attaatttga aaaagaaaaa catttattca attcgtaaac taggtgtagg
     3181 tattgcatct gtaactttag gtacattact tatatctggt ggcgtaacac ctgctgcaaa
     3241 tgctgcgcaa cacgatgaag ctcaacaaaa tgctttttat caagtcttaa atatgcctaa
     3301 cttaaatgct gatcaacgca atggttttat ccaaagcctt aaagatgatc caagccaaag
     3361 tgctaacgtt ttaggtgaag ctcaaaaact taatgactct caagctccaa aagctgatgc
     3421 gcaacaaaat aacttcaaca aagatcaaca aagcgccttc tatgaaatct tgaacatgcc
     3481 taacttaaac gaagcgcaac gtaacggctt cattcaaagt cttaaagacg acccaagcca
     3541 aagcactaac gttttaggtg aagctaaaaa attaaacgaa tctcaagcac cgaaagctga
     3601 taacaatttc aacaaagaac aacaaaatgc tttctatgaa atcttgaata tgcctaactt
     3661 aaacgaagaa caacgcaatg gtttcatcca aagcttaaaa gatgacccaa gccaaagtgc
     3721 taacctattg tcagaagcta aaaagttaaa tgaatctcaa gcaccgaaag cggataacaa
     3781 attcaacaaa gaacaacaaa atgctttcta tgaaatctta catttaccta acttaaacga
     3841 agaacaacgc aatggtttca tccaaagcct aaaagatgac ccaagccaaa gcgctaacct
     3901 tttagcagaa gctaaaaagc taaatgatgc tcaagcacca aaagctgaca acaaattcaa
     3961 caaagaacaa caaaatgctt tctatgaaat tttacattta cctaacttaa ctgaagaaca
     4021 acgtaacggc ttcatccaaa gccttaaaga cgatccttca gtgagcaaag aaattttagc
     4081 agaagctaaa aagctaaacg atgctcaagc accaaaagag gaagacaata acaagcctgg
     4141 caaagaagac aataacaagc ctggcaaaga agacaacaac aagcctggta aagaagacaa
     4201 caacaagcct ggcaaagaag acggcaacaa gcctggtaaa gaagacaaca aaaaacctgg
     4261 taaagaagat ggcaacaagc ctggtaaaga agacaacaaa aaaacctggt aaagaagacg
     4321 gcaacaagcc tggcaaagaa gatggcaaca aacctggtaa agaagatggt aacggagtac
     4381 atgtcgttaa acctggtgat acagtaaatg acattgcaaa agcaaacggc actactgctg
     4441 acaaaattgc aagcctatcg gaggtaggaa ttcgagctcg gtacccgggg atcctctaga
     4501 gtcgacctgc aggcatgcaa gctagcttaa gtaagtaagt aagccgccag ttccgctggc
     4561 ggcatttttt ttgatatcat cgatacaata cacaacgata gatatcattt tatccaaacc
     4621 aattttaact tatatacgtt gattaacaca ttcttatttg aaatgataag aatcatctaa
     4681 atgcacgagc aacatctttt gttgctcagt gcacatatgg tgcactctca gtacaatctg
     4741 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4801 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4861 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4921 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4981 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5041 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5101 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5161 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5221 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5281 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5341 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5401 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5461 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5521 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5581 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5641 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5701 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5761 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5821 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5881 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5941 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6001 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6241 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     6301 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6361 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6421 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6481 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6541 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6601 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6661 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6721 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6781 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6841 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6901 agagcctgtt agcacttata ttttgttatt gttcttcctc gatttcgtct atcattttgt
     6961 gattaatttc tcttttttct tgttctgtta agtcataaag ttcactagct aaatactctt
     7021 tttgtttcca aatataaaaa atttgataga tatattacgg ttg
//
